Automatic Nozzle Priming

This picture shows the automatic nozzle priming operation. Here, it is flushing the nozzles of G amidite that will be used for synthesis, in this case all eight nozzles. The blue tick mark at the top shows which reagent is being primed, the green blocks show which nozzles will be used for each reagent. x0 and x1 stand for Aux0 and Aux1, placeholders for two variable bases that can be used in addition to G, C, A, or T.

Priming is required to ensure that only fresh reagent or amidite is filling the delivery lines from bottle to nozzle tip. ACN is used to flush nozzles when synthesis is complete, and so it is neccessary to remove it before starting again.

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